ASCP ASCP-MLT Exam : MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)

ASCP-MLT
  • Exam Code: ASCP-MLT
  • Exam Name: MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
  • Updated: Aug 16, 2026
  • Q & A: 562 Questions and Answers

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ASCP ASCP-MLT Exam Syllabus Topics:

SectionObjectives
Topic 1: Microbiology- Clinical microbiological techniques
  • 1. Culture and sensitivity testing
    • 2. Bacteriology and organism identification
      Topic 2: Blood Banking / Immunohematology- Blood group systems and antibody identification
      • 1. Crossmatching procedures
        • 2. ABO and Rh systems
          Topic 3: Urinalysis and Body Fluids- Fluid analysis and interpretation
          • 1. Urine chemical and microscopic examination
            • 2. CSF and other body fluids
              Topic 4: Hematology- Blood cell analysis and disorders
              • 1. CBC and differential interpretation
                • 2. Anemias and leukemias overview
                  Topic 5: Clinical Chemistry- Metabolic and analytical testing
                  • 1. Enzymes and electrolytes
                    • 2. Endocrine and metabolic panels
                      Topic 6: Laboratory Operations- Quality and safety in the clinical lab
                      • 1. Quality control and assurance
                        • 2. Laboratory safety and regulations

                          ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

                          1. A normal hemoglobin molecule consists of a tetramer, of four heme and four globin chains, with a molecular weight of 64,500 daltons. Each of the four units can bind a molecule of oxygen for transport to the body's tissues. In the image shown below, there are four monomers (2 red globin chains and 2 blue globin chains) which form the entire hemoglobin tetramer structure. The green portions represent the 4 heme groups.
                          A normal hemoglobin molecule is comprised of the following:

                          A) Four heme and one globin chains
                          B) Ferrous iron and four globin chains
                          C) Four heme and four globin chains
                          D) One heme and four globin chains


                          2. AABB Standards do not require a DAT, autologous control, or a minor crossmatch for pretransfusion testing.
                          Essential components of compatibility testing include all of the following except :

                          A) Antibody screen on recipients serum
                          B) ABO and Rh typing of recipient
                          C) Major crossmatch or computer crossmatch
                          D) Direct Antiglobulin Test (DAT)


                          3. Autologous units must be drawn before they are needed, and must be readily available, therefore are generally not of use in emergencies.
                          All of the following are benefits of autologous donation except:

                          A) Reduces exposure to infectious agents
                          B) Are always on hand in case of an unexpected emergency
                          C) Eliminates sensitization to cellular blood components
                          D) Reduces demand for homologous blood


                          4. Considering the reactions given in this case study, Yersinia enterocolitica would be the best choice.
                          Most strains of Plesiomonas shigelloides, Escherichia coli, and Pasteurella multocida are urease negative.
                          Also, Plesiomonas shigelloides demonstrates a K/A reaction on a TSI slant.
                          The stool specimen from a patient admitted to the hospital with symptoms of appendicitis has a culture with the following characteristics:
                          Gram-negative bacillus
                          Catalase positive
                          Urease positive
                          Weakly fermentative
                          TSI Slant is A/A
                          These reactions suggest that the organism is MOST likely:

                          A) Plesiomonas shigelloides
                          B) Pasteurella multocida
                          C) Escherichia coli
                          D) Yersinia enterocolitica


                          5. The antiglobulin test may be omitted from the serological crossmatch if the patient's antibody screen is negative and there is no history of detection of unexpected antibodies.
                          Blood Bank
                          What must be true for the antiglobulin phase of the serologic crossmatch to be omitted (i.e., immediate spin crossmatch is done)? Please select all correct answers

                          A) The antibody screen must be negative.
                          B) The blood is needed for surgery
                          C) There is no history of detection of unexpected antibodies
                          D) The patient has not been transfused within the past 24 hours.


                          Solutions:

                          Question # 1
                          Answer: C
                          Question # 2
                          Answer: D
                          Question # 3
                          Answer: B
                          Question # 4
                          Answer: D
                          Question # 5
                          Answer: A,C

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